Anti-Cytokeratin 16 antibody [LL025]

• Supplier: Abcam
• Catalog: ab8741
• Prices: $395.00
• Sizes: 500 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone: LL025
• Applications: IHC-F IHC-F IHC-P WB ICC/IF ICC/IF
• Species Reactivities: Sheep, Human, Pig
• Antigen: Synthetic peptide (Human)
• Description: Mouse Monoclonal
• Gene: KRT16
• Conjugate: Unconjugated

Product images

ab8741 staining Cytokeratin 16 in Human hyperproliferative skin lesion (ulcus)tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 10% Serum for 30 minutes at 21°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). The sample was incubated with primary antibody (1/50 in PBS + 0.5% Tween-20 + 0.5% BSA) at 21°C for 30 minutes. An undiluted HRP-conjugated goat polyclonal to mouse IgG was used as secondary antibody.See Abreview

ICC/IF image of ab8741 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8741, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ab8741 staining Cytokeratin 16 in sheep epithelial tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in acetone, permeabilized using Tween 20, blocked with 10% serum for 2 hours at 20°C and then incubated with ab8741 at a 1/40 for 12 hours at 4°C. The secondary used was ab98640 Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 650), used at a 1/500 dilution.See Abreview

Product References

Proteomic analysis of laser-captured paraffin-embedded tissues: a molecular - Proteomic analysis of laser-captured paraffin-embedded tissues: a molecular

Patel V, Hood BL, Molinolo AA, Lee NH, Conrads TP, Braisted JC, Krizman DB, Veenstra TD, Gutkind JS. Clin Cancer Res. 2008 Feb 15;14(4):1002-14.

Epidermolysis bullosa simplex (Dowling-Meara type) is a genetic disease - Epidermolysis bullosa simplex (Dowling-Meara type) is a genetic disease

Ishida-Yamamoto A, McGrath JA, Chapman SJ, Leigh IM, Lane EB, Eady RA. J Invest Dermatol. 1991 Dec;97(6):959-68.