Anti-Cytokeratin 19 antibody [A53-B/A2]
| Name | Anti-Cytokeratin 19 antibody [A53-B/A2] |
|---|---|
| Supplier | Abcam |
| Catalog | ab7754 |
| Prices | $404.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | A53-B/A2 |
| Applications | IHC-F ICC/IF ICC/IF FC WB IHC-P ICC/IF ELISA IP |
| Species Reactivities | Human |
| Antigen | Human mammary carcinoma cell line MCF-7 |
| Description | Mouse Monoclonal |
| Gene | KRT19 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-Cytokeratin 19 antibody [A53-B/A2] (ab7754) at 1/1000 dilutionLane 1 : Lung tumour lysate (patient 1)Lane 2 : Lung tumour lysate (patient 2)Lane 3 : Lung tumour lysate (patient 3)Lane 4 : Lung tumour lysate (patient 4)Lysates/proteins at 5 µg per lane.SecondaryGoat anti mouse HRP (H and L) at 1/40000 dilutionPerformed under reducing conditions.Observed band size : 44 kDa (why is the actual band size different from the predicted?)Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.See Abreview](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/5537_ab7754_1.jpg)
All lanes : Anti-Cytokeratin 19 antibody [A53-B/A2] (ab7754) at 1/1000 dilutionLane 1 : Lung tumour lysate (patient 1)Lane 2 : Lung tumour lysate (patient 2)Lane 3 : Lung tumour lysate (patient 3)Lane 4 : Lung tumour lysate (patient 4)Lysates/proteins at 5 µg per lane.SecondaryGoat anti mouse HRP (H and L) at 1/40000 dilutionPerformed under reducing conditions.Observed band size : 44 kDa (why is the actual band size different from the predicted?)Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.See Abreview
ab7754 staining Cytokeratin 19 in human skin.Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ab7754 staining Cytokeratin 19 in Human liver tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% milk for 30 minutes at 37°C; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/1000 in antibody diluent) for 1 hour at 37°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
![Overlay histogram showing MCF7 cells stained with ab7754 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7754, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/5540_Cytokeratin-19-Primary-antibodies-ab7754-20.jpg)
Overlay histogram showing MCF7 cells stained with ab7754 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7754, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Immunofluorescence analysis of 1 month hepato-differentiated Human dental pulp stem cells, staining Cytokeratin 19 with ab7754 at 1/60 dilution. A FITC-conjugated anti-mouse IgG was used as the secondary antibody.
ICC/IF image of ab7754 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab7754 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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