Anti-Cytokeratin 4 antibody [EP1599Y]

• Supplier: Abcam
• Catalog: ab51599
• Prices: $391.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1599Y
• Applications: ICC/IF ICC/IF ICC/IF IP WB IHC-P FC
• Species Reactivities: Human
• Antigen: Synthetic peptide corresponding to C terminal of human Cytokeratin 4
• Description: Rabbit Monoclonal
• Gene: KRT4
• Conjugate: Unconjugated

Product images

ICC/IF image of ab51599 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab51599 at 1/100 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-Cytokeratin 4 antibody [EP1599Y] (ab51599) at 1/1000 dilution + A431 cell lysate at 10 µgSecondaryGoat anti-rabbit HRP at 1/2000 dilution

Immunohistochemical staining of paraffin embedded human cervical carcinoma tissue using ab51599 (1/100). Goat anti-rabbit HRP (1/2000) used for secondary staining.Immunohistochemical staining of paraffin embedded human cervical carcinoma tissue using ab51599 (1/100). Goat anti-rabbit HRP (1/2000) used for secondary staining.

Product References

Proteomic profile of keratins in cancer of the gingivo buccal complex: - Proteomic profile of keratins in cancer of the gingivo buccal complex:

Fulzele A, Malgundkar SA, Govekar RB, Patil A, Kane SV, Chaturvedi P, D'Cruz AK, Zingde SM. J Proteomics. 2013 Oct 8;91:242-58.

Down-regulation of keratin 4 and keratin 13 expression in oral squamous cell - Down-regulation of keratin 4 and keratin 13 expression in oral squamous cell

Sakamoto K, Aragaki T, Morita K, Kawachi H, Kayamori K, Nakanishi S, Omura K, Miki Y, Okada N, Katsube K, Takizawa T, Yamaguchi A. Histopathology. 2011 Mar;58(4):531-42.

Recovery of multipotent progenitors from the peripheral blood of patients - Recovery of multipotent progenitors from the peripheral blood of patients

Bui KC, Senadheera D, Wang X, Hendrickson B, Friedlich P, Lutzko C. Am J Respir Crit Care Med. 2010 Feb 1;181(3):226-37. doi:

A case of primary combined neuroendocrine carcinoma with squamous cell carcinoma - A case of primary combined neuroendocrine carcinoma with squamous cell carcinoma

Mochizuki Y, Omura K, Sakamoto K, Nakanishi S, Satoh K, Marukawa E, Yamaguchi A. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Apr;109(4):e34-9. doi:

Comprehensive keratin profiling reveals different histopathogenesis of - Comprehensive keratin profiling reveals different histopathogenesis of

Aragaki T, Michi Y, Katsube K, Uzawa N, Okada N, Akashi T, Amagasa T, Yamaguchi A, Sakamoto K. Hum Pathol. 2010 Dec;41(12):1718-25.