Anti-Cytokeratin 5 + 6 + 18 antibody [LP34]
| Name | Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] |
|---|---|
| Supplier | Abcam |
| Catalog | ab49289 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | LP34 |
| Applications | ICC/IF ICC/IF IHC-F FC IHC-P |
| Species Reactivities | Human |
| Antigen | Detergent-insoluble fraction of psoriatic human epidermis |
| Description | Mouse Monoclonal |
| Gene | KRT5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab49289 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49289, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
ELP3 localises to mitochondria and actin-rich domains at edges of HeLa cells. - ELP3 localises to mitochondria and actin-rich domains at edges of HeLa cells.
Barton D, Braet F, Marc J, Overall R, Gardiner J. Neurosci Lett. 2009 May 8;455(1):60-4.
Defects in tongue papillae and taste sensation indicate a problem with - Defects in tongue papillae and taste sensation indicate a problem with
Gardiner J, Barton D, Vanslambrouck JM, Braet F, Hall D, Marc J, Overall R. Neuroscientist. 2008 Jun;14(3):240-50.