Anti-Cytokeratin 5 antibody
| Name | Anti-Cytokeratin 5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab53121 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA IHC-P FC ICC/IF ICC/IF IHC-F IHC-F WB |
| Species Reactivities | Mouse, Rat, Human, Pig |
| Antigen | A synthetic peptide derived from human Cytokeratin 5 |
| Description | Rabbit Polyclonal |
| Gene | KRT5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Cytokeratin 5 antibody (ab53121) at 1/300 dilutionLane 1 : HepG2 cell extractLane 2 : HepG2 cell extract with immunising peptide
ab53121 at 1/50 dilution staining Cytokeratin 5 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
ICC/IF image of ab53121 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53121, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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Hemizygous Le-Cre transgenic mice have severe eye abnormalities on some genetic - Hemizygous Le-Cre transgenic mice have severe eye abnormalities on some genetic
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Estrogenic modulation of uropathogenic Escherichia coli infection pathogenesis in - Estrogenic modulation of uropathogenic Escherichia coli infection pathogenesis in
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