ICC/IF image of ab93279 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93279, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabiit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279) at 1/5000 dilution + A431 cell lysate at 10 µg](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/5758_Cytokeratin-6-Primary-antibodies-ab93279-4.jpg)
Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279) at 1/5000 dilution + A431 cell lysate at 10 µg
Immunohistochemical analysis of paraffin-embedded Human squamous cervical carcinoma using ab93279 at 1/250 dilution.
Overlay histogram showing A431 cells stained with ab93279 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93279, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.