ab18586 staining Cytokeratin 6 in human breast gland tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with methanol and blocked with 10% serum for 5 minutes. The sample was incubated with primary antibody (1/25) for 1 hour. An HRP-conjugated rabbit polyclonal to mouse IgG was used undiluted as secondary antibody. Ultravision ONE detection system has been used.See Abreview
ab18586 staining Cytokeratin 6 in breast organoids derived from normal human breast tissue by Immunocytochemistry.Cells were fixed in methanol and blocked using 10% serum for 5 minutes at 25°C. Samples were then incubated with ab18586 at a 1/25 for 1 hour at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal.See Abreview
 used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/5769_Cytokeratin-6-Primary-antibodies-ab18586-7.jpg)
Overlay histogram showing A431 cells stained with ab18586 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18586, 2µg/1x106 cells ) for 30 min at 22°C. The secondary antibody used was a goat anti-mous DyLight® 488 (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.