All lanes : Anti-D4 GDI antibody [EPR14212(B)] (ab181252) at 1/10000 dilutionLane 1 : Jurkat lysateLane 2 : U937 lysateLane 3 : HL60 lysateLane 4 : Human spleen lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow Cytometrical analysis of HL60 cells labeling D4 GDI with ab181252 at 1/70 (red) compared to a negative control antibody (green).
Immunofluorescent analysis of HL60 cells labeling D4 GDI with ab181252 at 1/100 (green) with Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 and DAPI staining (blue).
Immunohistochemical analysis of paraffin embedded Human bone marrow tissue labeling D4 GDI with ab181252 at 1/500.
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling D4 GDI with ab181252 at 1/500.