All lanes : Anti-Dab1 (phospho Y232) antibody [EPR2247(2)] (ab126728) at 1/10000 dilutionLane 1 : untreated SH-SY5Y cell lysateLane 2 : pervanadate treated SH-SY5Y cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
ab126728, at a dilution of 1/10 staining Dab1 (phospho Y232) in permeabilized pervanadate-treated (red) or untreated (negative) (green) SH-SY5Y cells by Flow Cytometry. Using ab126728 preincubated with phospho-Dab1 Phospho (pY232) peptide (blue) or non-phospho-Dab1 Phospho (pY232) peptide (orange).
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD