Anti-DAB2IP antibody
| Name | Anti-DAB2IP antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab87811 |
| Prices | $385.00 |
| Sizes | 20 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP IHC-P |
| Species Reactivities | Human, Mouse, Rat, Rabbit, Guinea Pig, Chimpanzee, Monkey, Ape, Elephant |
| Antigen | Synthetic peptide corresponding to a region between residue 850 and 900 of human DAB2IP (CAQ10385 |
| Description | Rabbit Polyclonal |
| Gene | DAB2IP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-DAB2IP antibody (ab87811) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µg
Detection of DAB2IP in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87811 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot.Detection: Chemiluminescence with an exposure time of 3 seconds.
IHC image of ab87811 staining in normal human breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87811, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
BRCA1 is a negative modulator of the PRC2 complex. - BRCA1 is a negative modulator of the PRC2 complex.
Wang L, Zeng X, Chen S, Ding L, Zhong J, Zhao JC, Wang L, Sarver A, Koller A, Zhi J, Ma Y, Yu J, Chen J, Huang H. EMBO J. 2013 May 29;32(11):1584-97.
Oncogenic herpesvirus HHV-8 promotes androgen-independent prostate cancer growth. - Oncogenic herpesvirus HHV-8 promotes androgen-independent prostate cancer growth.
Mygatt JG, Singhal A, Sukumar G, Dalgard CL, Kaleeba JA. Cancer Res. 2013 Sep 15;73(18):5695-708.