All lanes : Anti-DAP13 antibody [EPR15867] (ab192607) at 1/1000 dilutionLane 1 : Human fetal heartLane 2 : Human skeletal muscleLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
All lanes : Anti-DAP13 antibody [EPR15867] (ab192607) at 1/5000 dilutionLane 1 : Human fetal kidney lysateLane 2 : Human fetal liver lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-DAP13 antibody [EPR15867] (ab192607) at 1/5000 dilution + Rat muscle at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-DAP13 antibody [EPR15867] (ab192607) at 1/1000 dilutionLane 1 : Mouse brainLane 2 : Rat brainLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of formalin fixed paraffin embedded Human pancreas labeling DAP13 with ab192607 at 1/600 (0.5μg/ml) dilution and prediluted HRP polymer to Rabbit/Mouse IgG as secondary.
Immunohistochemical analysis of formalin fixed paraffin embedded mouse liver labeling DAP13 with ab192607 at 1/600 (0.5μg/ml) dilution and prediluted HRP polymer to Rabbit/Mouse IgG as secondary.
Immunofluorescent analysis of Hela cells permeabilised in 0.1% Triton X-100 and fixed in 4% paraformaldehyde labeling DAP13 with ab192607 at 1/50 (5.9 μg/ml) and Goat anti rabbit IgG(Alexa Fluor® 488) ab150077. Counterstained with DAPI.