All lanes : Anti-DAP13 antibody [EPR15867-28] (ab192617) at 1/1000 dilutionLane 1 : mouse brain tissue lysateLane 2 : mouse heart tissue lysateLane 3 : mouse kidney tissue lysateLane 4 : mouse heart tissue lysateLane 5 : mouse kidney tissue lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunofluorescent analysis of HeLa cells (100% Methanol-fixed, 0.1% triton X-100-permealized) labeling DAP13 with ab192617 at 1/100 dilution (20 μg/ml). A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with Counterstain: DAPI.
Anti-DAP13 antibody [EPR15867-28] (ab192617) at 1/5000 dilution + Rat muscle tissue lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Anti-DAP13 antibody [EPR15867-28] (ab192617) at 1/5000 dilution + Human fetal heart tissue lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
All lanes : Anti-DAP13 antibody [EPR15867-28] (ab192617) at 1/1000 dilutionLane 1 : Human fetal heart tissue lysateLane 2 : Human skeletal muscle tissue lysateLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Rat muscle tissue labeling DAP13 with ab192617 at 1/1000 dilution (2μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling DAP13 with ab192617 at 1/1000 dilution (2μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling DAP13 with ab192617 at 1/1000 dilution (2μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.