ab2637 staining DAP3 in HeLa cells by Immunocytochemistry/ Immunofluorescence before(left) and after(right) si-RNA-mediateded DAP3 knockdown expression. ab2637 diluted to 0.5 μg/ml and incubated at room temperature for 1 hour. Nuclei stained with DAPI.
All lanes : Anti-DAP3 antibody (ab2637) at 1 µg/ml (for 1 hour)Lane 1 : HeLa cell lysate in RIPA buffer - controlLane 2 : HeLa cell lysate in RIPA buffer - after siRNA mediated DAP3 knock-down expressionLysates/proteins at 35 µg per lane.
All lanes : Anti-DAP3 antibody (ab2637)Lane 1 : HEK293 mock transfection cell lysateLane 2 : HEK293 overexpressing DAP3 cell lysate
Anti-DAP3 antibody (ab2637) at 3 µg/ml (for 1 hour) + HeLa cell lysate in RIPA buffer at 30 µg