Anti-DARC antibody
| Name | Anti-DARC antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58965 |
| Prices | $378.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Human, Rat |
| Antigen | A 16 amino acid peptide from near the carboxy terminus of human DARC |
| Description | Rabbit Polyclonal |
| Gene | ACKR1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lane 1 : Anti-DARC antibody (ab58965) at 0.5 µg/mlLane 2 : Anti-DARC antibody (ab58965) at 1 µg/mlLane 3 : Anti-DARC antibody (ab58965) at 2 µg/mlLane 1 : mouse brain tissue lysateLane 2 : mouse brain tissue lysateLane 3 : mouse brain tissue lysateLysates/proteins at 15 µg per lane.SecondaryLanes 1 - 2 : Anti-rabbit IgG HRP at 1/1000 dilutionLane 3 : Anti-rabbit IgG HRP at 1/10000 dilution
ICC/IF image of ab58965 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58965, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Mesenchymal contribution to recruitment, infiltration, and positioning of - Mesenchymal contribution to recruitment, infiltration, and positioning of
Samaniego R, Estecha A, Relloso M, Longo N, Escat JL, Longo-Imedio I, Aviles JA, del Pozo MA, Puig-Kroger A, Sanchez-Mateos P. J Invest Dermatol. 2013 Sep;133(9):2255-64.
Expression of chemokine decoy receptors and their ligands at the porcine - Expression of chemokine decoy receptors and their ligands at the porcine
Wessels JM, Linton NF, van den Heuvel MJ, Cnossen SA, Edwards AK, Croy BA, Tayade C. Immunol Cell Biol. 2011 Feb;89(2):304-13.