All lanes : Anti-DAZAP1 antibody [EPR14400] (ab182558) at 1/50000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling DAZAP1 with ab182558 at 1/10 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab182558 at 1/50 dilution (Lane 1). Lane 2: Negative control.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.