All lanes : Anti-DAZAP1 antibody [EPR14400-60] (ab184183) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : HepG2 cell lysateLane 4 : 293T cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Western blot analysis of DAZAP1 in Jurkat cell lysate immunoprecipitated using ab184183 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Jurkat lysate.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.