Anti-DCAMKL1 antibody
| Name | Anti-DCAMKL1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab37994 |
| Prices | $401.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF FC ELISA WB IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide corresponding to Human DCAMKL1 aa 690-720 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Rabbit Polyclonal |
| Gene | DCLK1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-DCAMKL1 antibody (ab37994) at 1/750 dilution (for 20 hours at 4°C) + Human brain whole tissue lysate at 22 µgSecondaryAn undiluted HRP-conjugated Goat anti-rabbit polyclonaldeveloped using the ECL techniquePerformed under reducing conditions.
Anti-DCAMKL1 antibody (ab37994) at 1/1000 dilution + Mouse heart tissue lysate at 35 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling DCAMKL1 with ab37994. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling DCAMKL1 with ab37994. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by AEC staining.
ICC/IF image of ab37994 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37994, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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