Anti-DCAMKL1 antibody
| Name | Anti-DCAMKL1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab31704 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-F IHC-P WB IP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Mouse DCAMLK1 |
| Blocking Peptide | Mouse DCAMKL1 peptide |
| Description | Rabbit Polyclonal |
| Gene | DCLK1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-DCAMKL1 antibody (ab31704) at 1 µg/ml + Mouse Brain Whole Tissue Lysate at 20 µgSecondaryIR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.
Anti-DCAMKL1 antibody (ab31704) at 1 µg/ml + Brain (Rat) Tissue Lysate - normal tissue at 10 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
Anti-DCAMKL1 antibody (ab31704) at 1 µg/ml + Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
![DCAMKL1 was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to DCAMKL1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31704.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 82kDa: DCAMKL1; non specific - 52 and 27kDa: We are unsure as to the identity of this extra band.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/6595_DCAMKL1-Primary-antibodies-ab31704-12.jpg)
DCAMKL1 was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to DCAMKL1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31704.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 82kDa: DCAMKL1; non specific - 52 and 27kDa: We are unsure as to the identity of this extra band.
ICC/IF image of ab31704 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31704, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
ICC/IF image of ab31704 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31704, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.
ab31704 at a 1/1000 dilution staining DCAMKL1 in rat brain tissue sections (hippocampus) by Immunohistochemistry/ PFA perfused, frozen sections, incubated for 18 hours at 20°C in PBS + 0.3% Triton X-100. Secondary used at 1/1000 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 488.The immunostaining was performed using the `free floating` technique, using direct fluorescence. The antibody beautifully stains tracts of fibers in many brain areas such as the cortex, striatum. The image shown is staining observed at the level of the striatum.See Abreview
Product References
Expression of LGR-5, MSI-1 and DCAMKL-1, putative stem cell markers, in the early - Expression of LGR-5, MSI-1 and DCAMKL-1, putative stem cell markers, in the early
Femia AP, Dolara P, Salvadori M, Caderni G. BMC Cancer. 2013 Feb 1;13:48.
Altered expression of a putative progenitor cell marker DCAMKL1 in the rat - Altered expression of a putative progenitor cell marker DCAMKL1 in the rat
Kikuchi M, Nagata H, Watanabe N, Watanabe H, Tatemichi M, Hibi T. BMC Gastroenterol. 2010 Jun 18;10:65.
Identification of a novel putative pancreatic stem/progenitor cell marker - Identification of a novel putative pancreatic stem/progenitor cell marker
May R, Sureban SM, Lightfoot SA, Hoskins AB, Brackett DJ, Postier RG, Ramanujam R, Rao CV, Wyche JH, Anant S, Houchen CW. Am J Physiol Gastrointest Liver Physiol. 2010 Aug;299(2):G303-10. doi: