Anti-DCAMKL1 antibody (ab88484) at 5 µg/ml + Partial tagged recombinant DCAMLK1 protein (immunogen) at 0.1 µg
Anti-DCAMKL1 antibody (ab88484) at 5 µg/ml + NIH/3T3 cell lysate at 50 µg
ab88484, at 10 µg/ml, stainining DCAMKL1 in NIH/3T3 cells by Immunofluorescence.
IHC image of ab88484 staining in Human Hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88484, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.