Anti-DCC antibody [AF5]
| Name | Anti-DCC antibody [AF5] |
|---|---|
| Supplier | Abcam |
| Catalog | ab16793 |
| Prices | $359.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | AF5 |
| Applications | IHC-P FC ICC/IF WB IP |
| Species Reactivities | Mouse, Human |
| Antigen | Immunogen used was the extracellular domain of the human DCC protein |
| Description | Mouse Monoclonal |
| Gene | DCC |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing UG-87 cells stained with ab16793 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16793, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/6627_DCC-Primary-antibodies-ab16793-1.jpg)
Overlay histogram showing UG-87 cells stained with ab16793 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16793, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
Autoantibodies to epilepsy-related LGI1 in limbic encephalitis neutralize - Autoantibodies to epilepsy-related LGI1 in limbic encephalitis neutralize
Ohkawa T, Fukata Y, Yamasaki M, Miyazaki T, Yokoi N, Takashima H, Watanabe M, Watanabe O, Fukata M. J Neurosci. 2013 Nov 13;33(46):18161-74.
Presenilin-dependent receptor processing is required for axon guidance. - Presenilin-dependent receptor processing is required for axon guidance.
Bai G, Chivatakarn O, Bonanomi D, Lettieri K, Franco L, Xia C, Stein E, Ma L, Lewcock JW, Pfaff SL. Cell. 2011 Jan 7;144(1):106-18.
Axon initiation and growth cone turning on bound protein gradients. - Axon initiation and growth cone turning on bound protein gradients.
Mai J, Fok L, Gao H, Zhang X, Poo MM. J Neurosci. 2009 Jun 10;29(23):7450-8.