ab45868 (5µg/ml) staining of DCDC2 in paraffin embedded Human Cerebral Cortex following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows cytoplasm staining in select neurons.
ab45868 staining rat optic nerve sections by IHC-FoFr. The animal was perfused with 4% paraformaldehyde and further post fixed with 4% paraformaldehyde overnight. The tissues were cryoprotected and sectioned using a cryostat. Staining with ab45868 at a 1/200 dilution in 0.3% TritonX with 0.1x PBS in 10% donkey serum was performed for 1h at 24°C. A donkey anti-goat Alexa488 polyclonal antibody at 1/1000 was used as the secondary antibody. β III tubulin can be observed in red (Alexa fluro 568 secondary). DCDC2 is expressed in the optic nerve.See Abreview
All lanes : Anti-DCDC2 antibody (ab45868) at 0.2 µg/mlLane 1 : COS7 cell lysate transfected with full length recombinant human DCDC2Lane 2 : Untransfected control COS7 cells
All lanes : Anti-DCDC2 antibody (ab45868) at 1/500 dilutionLane 1 : adult mouse heartLane 2 : adult mouse brainLane 3 : three-month old mouse brainLane 4 : 5 week old mouse brainLane 5 : mouse hippcampus including dentate gyrusLane 6 : mouse DRGLane 7 : primary mouse neuronal cultureLane 8 : mouse astrocyte cultureLysates/proteins at 20 µg per lane.SecondaryDAKO cytomation Conjugation: HRPdeveloped using the ECL techniquePerformed under reducing conditions.