Anti-Dcp2 antibody
| Name | Anti-Dcp2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab28658 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P EMSA IP |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Dcp2 |
| Description | Rabbit Polyclonal |
| Gene | DCP2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab28658 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab28658, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Image courtesy of Human Protein Atlasab28658 stainning Dcp2 in paraffin embedded human kidney. Kidney tissue sections (4 µm) were incubated with ab28658 (1/35 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.ab28658 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
All lanes : Anti-Dcp2 antibody (ab28658) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human Dcp2 peptide (ab30458) at 1 µg/mlLane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human Dcp2 peptide (ab30458) at 1 µg/mlLane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Human Dcp2 peptide (ab30458) at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
![Dcp2 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Dcp2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab28658.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 52,58kDa: Dcp2.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/6725_Dcp2-Primary-antibodies-ab28658-14.jpg)
Dcp2 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Dcp2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab28658.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 52,58kDa: Dcp2.
Product References
Phosphorylation of mRNA decapping protein Dcp1a by the ERK signaling pathway - Phosphorylation of mRNA decapping protein Dcp1a by the ERK signaling pathway
Chiang PY, Shen YF, Su YL, Kao CH, Lin NY, Hsu PH, Tsai MD, Wang SC, Chang GD, Lee SC, Chang CJ. PLoS One. 2013 Apr 18;8(4):e61697.
Tristetraprolin controls the stability of cIAP2 mRNA through binding to the 3'UTR - Tristetraprolin controls the stability of cIAP2 mRNA through binding to the 3'UTR
Kim CW, Kim HK, Vo MT, Lee HH, Kim HJ, Min YJ, Cho WJ, Park JW. Biochem Biophys Res Commun. 2010 Sep 10;400(1):46-52. doi: