All lanes : Anti-DCTN5 antibody [EPR11253] (ab157197) at 1/1000 dilutionLane 1 : MCF7 cell lysateLane 2 : Human fetal liver cell lysateLane 3 : SH-SY5Y cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DCTN5 with ab157197 at 1/100 dilution.
Immunofluorescent analysis of MCF7 cells labeling DCTN5 with ab157197 at 1/250 dilution.
Detection of DCTN5 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab156197 at 1/10 dilution.