Anti-DDB1 antibody [1322]
| Name | Anti-DDB1 antibody [1322] |
|---|---|
| Supplier | Abcam |
| Catalog | ab13562 |
| Prices | $388.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 1322 |
| Applications | FC ELISA WB |
| Species Reactivities | Human |
| Antigen | Synthetic peptide (Human) conjugated to a protein carrier |
| Description | Mouse Monoclonal |
| Gene | DDB1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab13562 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13562, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/6922_DDB1-Primary-antibodies-ab13562-4.jpg)
Overlay histogram showing HeLa cells stained with ab13562 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13562, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
The human immunodeficiency virus type 2 Vpx protein usurps the CUL4A-DDB1 DCAF1 - The human immunodeficiency virus type 2 Vpx protein usurps the CUL4A-DDB1 DCAF1
Bergamaschi A, Ayinde D, David A, Le Rouzic E, Morel M, Collin G, Descamps D, Damond F, Brun-Vezinet F, Nisole S, Margottin-Goguet F, Pancino G, Transy C. J Virol. 2009 May;83(10):4854-60.
Molecular architecture and assembly of the DDB1-CUL4A ubiquitin ligase machinery. - Molecular architecture and assembly of the DDB1-CUL4A ubiquitin ligase machinery.
Angers S, Li T, Yi X, MacCoss MJ, Moon RT, Zheng N. Nature. 2006 Oct 5;443(7111):590-3.