Western blot analysis on immunoprecipitation pellet from (1) Jurkat cells lysate or (2) 1X PBS (negative control) using ab176348 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Flow cytometric analysis of permeabilized Jurkat cells labeling DDX39 with ab176348 (red) or a rabbit IgG (negative) (green).
Immunofluorescent staining of HepG2 cells labeling DDX39 with ab176348 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DDX39 with ab176348 at 1/50 dilution.
All lanes : Anti-DDX39 antibody [EPR13507(B)] (ab176348) at 1/10000 dilutionLane 1 : Human seminoma lysateLane 2 : Jurkat cell lysateLane 3 : HeLa cell lysateLane 4 : HepG2 cell lysateLane 5 : A431 cell lysateLysates/proteins at 10 µg per lane.