Immunofluorescence analysis of HeLa cells (fixative -20℃ Acetone) labeling DDX41 with ab182007 at a 1/250 dilution. Goat anti rabbit IgG (Dylight 488) secondary used at a 1/200 diution.
Immunohistochemical analysis of paraffin embedded Human colon tissue labeling DDX41 with ab182007 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
Anti-DDX41 antibody [EPR14298] (ab182007) at 1/10000 dilution + Jar cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-DDX41 antibody [EPR14298] (ab182007) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : 293 cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution