Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DDX56 with ab115158 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation of DDX56 (3 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1-Immunoprecipitation with an anti-DDX56 antibody that recognizes an upstream epitope; Lane 2- Immunoprecipitation with anti-DDX56 antibody (ab115158); Lane 3- Immunoprecipitation with IgG control. Anti-DDX56 antibody (ab115158) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 30 sec.
All lanes : Anti-DDX56 antibody (ab115158) at 0.1 µg/mlLane 1 : HeLa cell lysate at 50 µgLane 2 : HeLa cell lysate at 15 µgLane 3 : HeLa cell lysate at 5 µgLane 4 : 293T cell lysate at 50 µg