Anti-DDX58 antibody
| Name | Anti-DDX58 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab45428 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Full length human DDX58 protein (N-terminal tag) |
| Description | Rabbit Polyclonal |
| Gene | DDX58 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lane 1 : Anti-DDX58 antibody (ab45428) at 0.5 µg/mlLane 2 : Anti-DDX58 antibody (ab45428) at 1 µg/mlLane 3 : Anti-DDX58 antibody (ab45428) at 2 µg/mlLane 1 : C2C12 cell lysateLane 2 : C2C12 cell lysateLane 3 : C2C12 cell lysate
ab45428 at 5 µg/ml staining DDX58 in human heart tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
ICC/IF image of ab45428 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45428, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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Degradation of RIG-I following cytomegalovirus infection is independent of - Degradation of RIG-I following cytomegalovirus infection is independent of
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