Anti-DEK antibody
| Name | Anti-DEK antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab22826 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ELISA ICC/IF ICC/IF |
| Species Reactivities | Human, Rat |
| Antigen | A 14-22 amino acid region within synthetic peptide: APAAEGEGTP TQPASEKEPE MPGPREESEE EEDEDDEEEE EEEKEKSLIV EGKRE, corresponding to N terminal amino acids 5-59 of Human DEK Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | DEK |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-DEK antibody (ab22826) at 0.4 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)SecondaryDonkey Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7083)
ab22826 at 4.0 - 8.0 ug/ml, staining human DEK in paraffin embedded liver tissue. Arrows indicate positively labelled cells (hepatocytes).
ab22826 at 4.0 - 8.0 ug/ml, staining human DEK in paraffin embedded thymus tissue. Arrows indicate positively labelled cells (spleen cells).
ICC/IF image of ab22826 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22826, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.