All lanes : Anti-DEK antibody [EPR11034] (ab166624) at 1/1000 dilutionLane 1 : 293T cell lysatesLane 2 : HeLa cell lysatesLane 3 : Jurkat cell lysatesLane 4 : K562 cell lysatesLysates/proteins at 10 µg per lane.
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling DEK with ab166624 at 1/250 dilution.
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling DEK with ab166624 at 1/250 dilution.
Immunofluorescence analysis of 293T cells labeling DEK with ab166624 at 1/100 dilution.
Immunoprecipitation of DEK in pellet from HeLa cell lysate using ab166624 at 1/10 dilution, followed by WB detection of DEK using as secondary an HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG.