Western blot against tagged recombinant protein immunogen using ab54750 Deoxyribonuclease I like 1 antibody at 1ug/ml.
ab54750 at a 1/50 dilution staining Deoxyribonuclease I like 1 in Dog macrophage cell line DH82 by Immunocytochemistry/ PFA fixed, incubated for 1 hour at 25°C. Blocked using 1% BSA for 30 minutes at 25°C. Secondary used at 1/100 polyclonal Goat anti-mouse conjugated to Alexa Fluor 488.See Abreview
Anti-Deoxyribonuclease I like 1 antibody (ab54750) at 1/1500 dilution (in TBS + 1% Skim milk + 0.05% Tween 20 for 12 hours at 4°C) + Whole cell lysate of a Human acute leukaemia cell line at 1000000 cellsSecondaryAn HRP-conjugated Goat anti-mouse polyclonal at 1/2000 dilutiondeveloped using the ECL techniqueObserved band size : ~42 kDa (why is the actual band size different from the predicted?)Exposure time : 50 secondsThis image is courtesy of an Abreview submitted by Dipu Mohan KumarSee Abreview