Anti-DEP1 antibody
| Name | Anti-DEP1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab10639 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | Synthetic peptide derived from residues 1200 - 1300 of Human DEP1 |
| Description | Rabbit Polyclonal |
| Gene | PTPRJ |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Recombinant PTP phosphatase domains (5µg per lane) were probed with ab10639 at 1/500. The antibody recognises the recombinant DEP1(PTPRJ) fusion protein at ~60 kDa.Lane 1: DEP1 (PTPRJ)Lane 2: GLEPP1 (PTPRO)Lane 3: PTP beta (PTPRB)Lane 4: PTP sigma (PTPRS)Lane 5: PCPTP1 (PTPRR)
Liver carcinoma tissue was fixed in 10% formalin at 4°C overnight and paraffin embedded. Following blocking the tissue sections were incubated with ab10639 (1/100 dilution) at 37°C for 1 hour, and with the secondary antibody at 37°C for 30 minutes. The slides were counterstained with Hematoxylin. Washes were carried out using TBS. Heat mediated antigen retrieval was carried out using the Microwave method before commencing the staining protocol. ab10639 is clearly localised to the cytoplasm of the tissue (brown). Nuclear staining can be seen in blue. 100x magnification.