Anti-Destrin antibody [EPR15827(B)]

• Supplier: Abcam
• Catalog: ab186754
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR15827(B)
• Applications: ICC/IF ICC/IF WB IHC-P FC IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Destrin aa 50-150
• Description: Rabbit Monoclonal
• Gene: DSTN
• Conjugate: Unconjugated

Product images

Anti-Destrin antibody [EPR15827(B)] (ab186754) at 1/10000 dilution + MCF-7 cell lyate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

All lanes : Anti-Destrin antibody [EPR15827(B)] (ab186754) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : A549 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

All lanes : Anti-Destrin antibody [EPR15827(B)] (ab186754) at 1/2000 dilutionLane 1 : C6 cell lysateLane 2 : PC-12 cell lysateLane 3 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling Destrin with ab186754 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Destrin with ab186754 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF-7 cells labeling Destrin with ab186754 at 1/1000 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

Flow cytometric analysis of 2% paraformaldehyde-fixed MCF-7 cells labeling Destrin with ab186754 at 1/360 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Western blot analysis of Destrin in A549 cell lysate immunoprecipitated with ab186754 at 1/50 dilution. Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.