Anti-Destrin antibody [EPR15828]

• Supplier: Abcam
• Catalog: ab192262
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR15828
• Applications: WB IHC-P ICC/IF ICC/IF IP
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Destrin aa 100 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: DSTN
• Conjugate: Unconjugated

Product images

All lanes : Anti-Destrin antibody [EPR15828] (ab192262) at 1/5000 dilutionLane 1 : MCF-7 cell lysateLane 2 : HepG2 cell lysateLane 3 : HeLa cell lysateLane 4 : A549 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin embedded human cervical carcinoma  tissue sections labeling Destrin using ab192262 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Immunofluorescent analysis of 4% formaldehyde fixed MCF-7 cells labeling Destrin using ab192262 at a 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) ab150077 was used as the secondary at a 1/200 dilution. Permeabilized using 0.1% Triton X-100. The two negative controls; 1. Primary ab concentration (anti-Destrin ) is 1:50 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1:500 dilution; 2. Primary ab concentration (anti-Destrin ) is 1:50 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1:500 dilution.

Lysate from A549 cells was immunoprecipitated with ab192262 at a 1/30 dilution. For the subsequent blot, ab192262 used at a 1/1000 dilution with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.