Anti-DFNA5 antibody - N-terminal (ab175614) at 1/1000 dilution + HeLa cell lysate at 35 µg
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human breast carcinoma tissue, labeling DFNA5 using ab175614 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometry analysis of MDA-MB468 cells labeling DFNA5 (green, bottom histogram), using ab175614 at a 1/10 dilution, and negative control cells (blue, top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.