All lanes : Anti-DGKA antibody [3G7] (ab119825) at 1/500 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with pCMV6-ENTRY DGKA Lysates/proteins at 5 µg per lane.
All lanes : Anti-DGKA antibody [3G7] (ab119825) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : SVT2 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.
Flow cytometric analysis of Jurkat cells, using ab119825, at 1/100 (Red) compared to a nonspecific negative control antibody (Blue).