Anti-Dicer antibody [13D6] - ChIP Grade
| Name | Anti-Dicer antibody [13D6] - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab14601 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | 13D6 |
| Applications | ICC/IF IP ICC/IF ICC/IF IHC-P WB EMSA ChIP FC |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide (Human) |
| Description | Mouse Monoclonal |
| Gene | DICER1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HEK293 cells stained with ab14601 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14601, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/8360_ab14601-7-ab14601FC.jpg)
Overlay histogram showing HEK293 cells stained with ab14601 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14601, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Mouse embryonic fibroblasts (MEFs) were fixed in 4%PFA, permeabilised in PBS 1%BSA 0.1%TX-100 and incubated with anti-Dicer antibody ab14601 (1:100 dilution) o/n. The antibody showed mainly cytoplasmic staining.
HepG2 cell lysate at 20 µgdeveloped using the ECL techniquePerformed under reducing conditions.Additional bands at : 100 kDa (possible non-specific binding),60 kDa (possible non-specific binding).
ChIP analysis of Dicer protein binding at chicken ß-globin regulatory elements. Chromatin from human erythroleukemia (K562) cells containing chicken chromosomes with normal (FRK2) or mutant (HS1-K) ß-globin loci and chicken DT40 cells was immunprecipitated with antibodies to Dicer (ab14601). PCR analysis of immunoprecipitated chromatin was carried out using primers to the chicken ß-globin regulatory elements. Mouse IgG served as control. 10% of input DNA was utilized.
![All lanes : Anti-Dicer antibody [13D6] - ChIP Grade (ab14601) at 2.5 µg/mlLane 1 : human B cell lymphoma lysateLane 2 : human B cell lymphoma lysate infected with control shRNA retrovirusLane 3 : human B cell lymphoma lysate infected with Dicer knockdown shRNA virusLysates/proteins at 2.5 µg/ml per lane.Observed band size : 225 kDa (why is the actual band size different from the predicted?)Jing Huang and Elliot Epner Oregon Health and Science University](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/8364_ab14601_7.jpg)
All lanes : Anti-Dicer antibody [13D6] - ChIP Grade (ab14601) at 2.5 µg/mlLane 1 : human B cell lymphoma lysateLane 2 : human B cell lymphoma lysate infected with control shRNA retrovirusLane 3 : human B cell lymphoma lysate infected with Dicer knockdown shRNA virusLysates/proteins at 2.5 µg/ml per lane.Observed band size : 225 kDa (why is the actual band size different from the predicted?)Jing Huang and Elliot Epner Oregon Health and Science University
ab14601 staining Dicer in mouse ear tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in sodium citrate and then blocking with 5% serum was performed for 2 hours at 25°C. The primary antibody was diluted 1/100 and incubated with sample for 20 hours at 4°C. An Alexa Fluor®488-conjugated goat polyclonal to mouse IgG was used at dilution at 1/200 as secondary antibody.See Abreview
Product References
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Identification and characterization of Dicer1e, a Dicer1 protein variant, in oral - Identification and characterization of Dicer1e, a Dicer1 protein variant, in oral
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Germ-line and somatic DICER1 mutations in pineoblastoma. - Germ-line and somatic DICER1 mutations in pineoblastoma.
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