Anti-Dihydrofolate reductase (DHFR) antibody
| Name | Anti-Dihydrofolate reductase (DHFR) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab82171 |
| Prices | $378.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | A synthetic peptide corresponding to internal region of human DHFR |
| Description | Rabbit Polyclonal |
| Gene | DHFR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Formalin-fixed, paraffin-embedded section of Human breast carcinoma stained with ab82171 at 1/50 dilution. Incubate primary antibody (ab82171) for 30 minutes at RT.
ICC/IF image of ab82171 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82171, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.