Anti-DLK antibody
| Name | Anti-DLK antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab21682 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB |
| Species Reactivities | Mouse, Human, Rat, Bovine |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human DLK |
| Blocking Peptide | DLK peptide |
| Description | Rabbit Polyclonal |
| Gene | DLK1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-DLK antibody (ab21682) at 1 µg/mlLane 1 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate (ab27193)Lane 3 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
All lanes : Anti-DLK antibody (ab21682) at 1 µg/mlLane 1 : Human Embryonic Stem Cell LysateLane 2 : Mouse Embryonic Stem Cell LysateLane 3 : Human Embryonic Stem Cell Lysate with DLK peptide (ab23442) at 1 µg/mlLane 4 : Mouse Embryonic Stem Cell Lysate with DLK peptide (ab23442) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryRabbit IgG secondary antibody (ab28446) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Image courtesy of Human Protein Atlasab21682 staining DLK in human bone marrow within the haemopoietic tissue and not the adipocytes. Paraffin embedded human bone marrow was incubated with ab21682 (1/150 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab21682 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.
Product References
PKA regulatory subunit R2B is required for murine and human adipocyte - PKA regulatory subunit R2B is required for murine and human adipocyte
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