All lanes : Anti-DLK antibody [EPR3486(2)] (ab110636) at 1/5000 dilution (purified)Lane 1 : JAR cell lysateLane 2 : SH-SY5Y cell lysateLane 3 : NCCIT cell lysateLane 4 : A549 cell lysateLysates/proteins at 1/5000 dilution per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
All lanes : Anti-DLK antibody [EPR3486(2)] (ab110636) at 1/5000 dilution (purified)Lane 1 : Mouse brain tissue lysateLane 2 : Rat brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
All lanes : Anti-DLK antibody [EPR3486(2)] (ab110636) at 1/1000 dilution (unpurified)Lane 1 : JAR cell lysateLane 2 : SH SY5Y cell lysateLane 3 : NCCIT cell lysateLane 4 : A549 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution