All lanes : Anti-DLK2 antibody - C-terminal (ab176003) at 1/1000 dilutionLane 1 : Non-transfected 293 cell lysateLane 2 : 293 cell lysate transiently transfected with the DLK2 geneLysates/proteins at 2 µg per lane.
Flow cytometric analysis of HL60 cells labeling DLK2 with ab176003 at 1/10 dilution (green) compared to negative control cells (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling DLK2 with ab176003 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Anti-DLK2 antibody - C-terminal (ab176003) at 1/1000 dilution + HL60 cell lysate at 35 µg