Anti-DMGDH antibody [EPR16908]

• Supplier: Abcam
• Catalog: ab198292
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16908
• Applications: IP IHC-P WB
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DMGDH aa 850 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: DMGDH
• Conjugate: Unconjugated

Product images

All lanes : Anti-DMGDH antibody [EPR16908] (ab198292) at 1/10000 dilutionLane 1 : Human fetal kidney lysateLane 2 : Human fetal liver lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilutiondeveloped using the ECL technique

Anti-DMGDH antibody [EPR16908] (ab198292) at 1/1000 dilution + HepG2 (Human liver hepatocellular carcinoma) cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilutiondeveloped using the ECL technique

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DMGDH with ab198292 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. Inset image: negative control obtained using PBS instead of ab198292 and secondary antibody only.Note: Cytoplasm staining on human kidney tissue was observed.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling DMGDH with ab198292 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. Inset image: negative control obtained using PBS instead of ab198292 and secondary antibody only.Note: Cytoplasm staining on human liver tissue was observed.

DMGDH protein was immunoprecipitation from 1mg of Human fetal liver whole cell lysate with ab198292 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab198292 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution. Lane 1: Input, Human fetal liver whole cell lysate, 10ug. Lane 2: IP of DMGDH from Human fetal liver whole cell lysate. Lane 3: IP using Rabbit monoclonal IgG (ab172730) instead of ab198292 in Human fetal liver whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.