Anti-DNA PKcs antibody
| Name | Anti-DNA PKcs antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab230 |
| Prices | $384.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB IP EMSA |
| Species Reactivities | Mouse, Rat, Hamster, Human |
| Antigen | Synthetic peptide (Human) |
| Description | Rabbit Polyclonal |
| Gene | PRKDC |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab230 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab230, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Discovery of DNA repair inhibitors by combinatorial library profiling. - Discovery of DNA repair inhibitors by combinatorial library profiling.
Moeller BJ, Sidman RL, Pasqualini R, Arap W. Cancer Res. 2011 Mar 1;71(5):1816-24.
Regulation of cell proliferation by interleukin-3-induced nuclear translocation - Regulation of cell proliferation by interleukin-3-induced nuclear translocation
Hoshino A, Hirst JA, Fujii H. J Biol Chem. 2007 Jun 15;282(24):17706-11. Epub 2007 Apr 19.
Human chronic lymphocytic leukemia B cells can escape DNA damage-induced - Human chronic lymphocytic leukemia B cells can escape DNA damage-induced
Deriano L, Guipaud O, Merle-Beral H, Binet JL, Ricoul M, Potocki-Veronese G, Favaudon V, Maciorowski Z, Muller C, Salles B, Sabatier L, Delic J. Blood. 2005 Jun 15;105(12):4776-83. Epub 2005 Feb 17.
Valosin-containing protein phosphorylation at Ser784 in response to DNA damage. - Valosin-containing protein phosphorylation at Ser784 in response to DNA damage.
Livingstone M, Ruan H, Weiner J, Clauser KR, Strack P, Jin S, Williams A, Greulich H, Gardner J, Venere M, Mochan TA, DiTullio RA Jr, Moravcevic K, Gorgoulis VG, Burkhardt A, Halazonetis TD. Cancer Res. 2005 Sep 1;65(17):7533-40.
DNA double strand break repair in human bladder cancer is error prone and - DNA double strand break repair in human bladder cancer is error prone and
Bentley J, Diggle CP, Harnden P, Knowles MA, Kiltie AE. Nucleic Acids Res. 2004 Oct 5;32(17):5249-59. Print 2004.
Development of a rapid, small-scale DNA repair assay for use on clinical samples. - Development of a rapid, small-scale DNA repair assay for use on clinical samples.
Diggle CP, Bentley J, Kiltie AE. Nucleic Acids Res. 2003 Aug 1;31(15):e83.