Anti-DNA Polymerase beta antibody [61]
| Name | Anti-DNA Polymerase beta antibody [61] |
|---|---|
| Supplier | Abcam |
| Catalog | ab1831 |
| Prices | $390.00 |
| Sizes | 250 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | 61 |
| Applications | WB IHC-P IHC-F ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Human |
| Antigen | BALB/C mice were injected with DNA polymerase beta protein |
| Description | Mouse Monoclonal |
| Gene | POLB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab1831 staining human testis by IHC-P.
![Overlay histogram showing Jurkat cells stained with ab1831 (red line). The cells were fixed with 4% paraformaldehyde and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1831, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/9040_DNA-Polymerase-beta-Primary-antibodies-ab1831-2.jpg)
Overlay histogram showing Jurkat cells stained with ab1831 (red line). The cells were fixed with 4% paraformaldehyde and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1831, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
![All lanes : Anti-DNA Polymerase beta antibody [61] (ab1831)Lane 1 : Empty vectorLane 2 : Wild-type mouse embryonic fibroblastsLane 3 : G231D mutant mouse embryonic fibroblastsLane 4 : T292I mutant mouse embryonic fibroblastsLane 5 : S275N/E295K mutant mouse embryonic fibroblasts](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/9041_DNA-Polymerase-beta-Primary-antibodies-ab1831-3.jpg)
All lanes : Anti-DNA Polymerase beta antibody [61] (ab1831)Lane 1 : Empty vectorLane 2 : Wild-type mouse embryonic fibroblastsLane 3 : G231D mutant mouse embryonic fibroblastsLane 4 : T292I mutant mouse embryonic fibroblastsLane 5 : S275N/E295K mutant mouse embryonic fibroblasts
Product References
K-Lysine acetyltransferase 2a regulates a hippocampal gene expression network - K-Lysine acetyltransferase 2a regulates a hippocampal gene expression network
Stilling RM, Ronicke R, Benito E, Urbanke H, Capece V, Burkhardt S, Bahari-Javan S, Barth J, Sananbenesi F, Schutz AL, Dyczkowski J, Martinez-Hernandez A, Kerimoglu C, Dent SY, Bonn S, Reymann KG, Fischer A. EMBO J. 2014 Sep 1;33(17):1912-27.
Y265C DNA polymerase beta knockin mice survive past birth and accumulate base - Y265C DNA polymerase beta knockin mice survive past birth and accumulate base
Senejani AG, Dalal S, Liu Y, Nottoli TP, McGrath JM, Clairmont CS, Sweasy JB. Proc Natl Acad Sci U S A. 2012 Apr 24;109(17):6632-7. doi:
Human POLB gene is mutated in high percentage of colorectal tumors. - Human POLB gene is mutated in high percentage of colorectal tumors.
Donigan KA, Sun KW, Nemec AA, Murphy DL, Cong X, Northrup V, Zelterman D, Sweasy JB. J Biol Chem. 2012 Jul 6;287(28):23830-9.
Colon cancer-associated DNA polymerase beta variant induces genomic instability - Colon cancer-associated DNA polymerase beta variant induces genomic instability
Nemec AA, Donigan KA, Murphy DL, Jaeger J, Sweasy JB. J Biol Chem. 2012 Jul 6;287(28):23840-9.
Two novel determinants of etoposide resistance in small cell lung cancer. - Two novel determinants of etoposide resistance in small cell lung cancer.
Lawson MH, Cummings NM, Rassl DM, Russell R, Brenton JD, Rintoul RC, Murphy G. Cancer Res. 2011 Jul 15;71(14):4877-87.
DNA polymerase beta is critical for mouse meiotic synapsis. - DNA polymerase beta is critical for mouse meiotic synapsis.
Kidane D, Jonason AS, Gorton TS, Mihaylov I, Pan J, Keeney S, de Rooij DG, Ashley T, Keh A, Liu Y, Banerjee U, Zelterman D, Sweasy JB. EMBO J. 2010 Jan 20;29(2):410-23.
The E295K DNA polymerase beta gastric cancer-associated variant interferes with - The E295K DNA polymerase beta gastric cancer-associated variant interferes with
Lang T, Dalal S, Chikova A, DiMaio D, Sweasy JB. Mol Cell Biol. 2007 Aug;27(15):5587-96. Epub 2007 May 25.
Expression of DNA polymerase {beta} cancer-associated variants in mouse cells - Expression of DNA polymerase {beta} cancer-associated variants in mouse cells
Sweasy JB, Lang T, Starcevic D, Sun KW, Lai CC, Dimaio D, Dalal S. Proc Natl Acad Sci U S A. 2005 Oct 4;102(40):14350-5. Epub 2005 Sep 22.