Anti-DNA Polymerase delta, catalytic subunit antibody [607]
| Name | Anti-DNA Polymerase delta, catalytic subunit antibody [607] |
|---|---|
| Supplier | Abcam |
| Catalog | ab10362 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | 607 |
| Applications | WB IP |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant protein, corresponding to amino acids 1-140 of the large subunit of Human DNA Polymerase delta |
| Description | Mouse Monoclonal |
| Gene | POLD1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
DNA Polymerase delta, catalytic subunit was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to DNA Polymerase delta, catalytic subunit and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab10362.Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.Band: 130kDa: DNA Polymerase delta, catalytic subunit
Product References
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