All lanes : Anti-DNA Polymerase delta, catalytic subunit antibody [EPR15118] (ab186407) at 1/10000 dilutionLane 1 : MOLT4 cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLane 4 : K562 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
All lanes : Anti-DNA Polymerase delta, catalytic subunit antibody [EPR15118] (ab186407) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : Raw264.7 cell lysateLane 3 : PC12 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling DNA Polymerase delta, catalytic subunit with ab186407 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling DNA Polymerase delta, catalytic subunit with ab186407 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling DNA Polymerase delta, catalytic subunit with ab186407 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
Immunofluorescent analysis of acetone-fixed K562 cells labeling DNA Polymerase delta, catalytic subunit with ab186407 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).