Anti-DNA polymerase eta antibody

• Supplier: Abcam
• Catalog: ab17725
• Prices: $392.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF ICC/IF IHC-P
• Species Reactivities: Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human DNA polymerase eta
• Blocking Peptide: Human DNA polymerase eta peptide
• Description: Rabbit Polyclonal
• Gene: POLH
• Conjugate: Unconjugated

Product images

Lanes 1, 2, 5 & 6 : Anti-DNA polymerase eta antibody (ab17725) at 1/400 dilutionLanes 3 - 4 : Anti-DNA polymerase eta antibody (ab17725) at 1/2000 dilutionLane 1 : XP30RO cell lysate (lacking endogenous DNA polymerase eta)Lane 2 : MRC5 cell lysateLane 3 : XP30RO cell lysate (lacking endogenous DNA polymerase eta)Lane 4 : MRC5 cell lysateLane 5 : XP30RO cell lysate(lacking endogenous DNA polymerase eta) with Human DNA polymerase eta peptide (ab19077) at 5 µlLane 6 : MRC5 cell lysate with Human DNA polymerase eta peptide (ab19077) at 5 µlSecondaryGoat anti-rabbit HRP at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

IHC image of DNA polymerase eta staining in human tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17725, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

All lanes : Anti-DNA polymerase eta antibody (ab17725) at 2 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human DNA polymerase eta peptide (ab19077) at 2 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

All lanes : Anti-DNA polymerase eta antibody (ab17725) at 2 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate blocked with 5% milkLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate blocked with 3% milkLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Product References

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