Atg5 (D5F5U) Rabbit mAb
| Name | Atg5 (D5F5U) Rabbit mAb |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 12994 |
| Prices | $99.00, $246.00 |
| Sizes | 20 µl (2 western blots), 100 µl (10 western blots) |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | D5F5U |
| Applications | WB IP |
| Species Reactivities | Human, Mouse, Rat, Monkey, Xenopus, Dog, Pig |
| Antigen | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu265 of human Atg5 protein. |
| Description | Rabbit Monoclonal |
| Gene | ATG5 |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from various cell lines using Atg5 (D5F5U) Rabbit mAb.
Western blot analysis of extracts from wild-type MEFs (wt) or MEFs from Atg5 knockouts (Atg5 -/- ) using Atg5 (D5F5U) Rabbit mAb (upper), or β-Actin (D6A8) Rabbit mAb #8457 (lower). Atg5 -/- MEFs were kindly provided by Dr. Ramnik Xavier, Massachusetts General Hospital, Harvard Medical School, Boston, MA.
Immunoprecipitation of Atg5 from PANC-1 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Atg5 (D5F5U) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Atg5 (D5F5U) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody to avoid cross-reactivity with rabbit IgG.