All lanes : Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : 293T cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
Immunofluorescent analysis of HepG2 cells labeling DNAJC7 with ab179830 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling DNAJC7 with ab179830 at 1/50 dilution.
Western blot analysis on immunoprecipitation pellet from (1) 293T cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab179830 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Flow cytometric analysis of permeabilized HeLa cells labeling DNAJC7 with ab179830 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling DNAJC7 with ab179830 at 1/50 dilution.