Anti-Dnmt1 antibody

• Supplier: Abcam
• Catalog: ab19905
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF ICC/IF IP IHC-P
• Species Reactivities: Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Dnmt1
• Blocking Peptide: Human Dnmt1 peptide
• Description: Rabbit Polyclonal
• Gene: DNMT1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Dnmt1 antibody (ab19905) at 1 µg/mlLane 1 : HeLa nuclear lysateLane 2 : HeLa nuclear lysate with Human Dnmt1 peptide (ab21999) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilutionPerformed under reducing conditions.

Dnmt1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Dnmt1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab19905.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 183kDa: Dnmt1.

ab19905 at a 1/200 dilution staining paraformaldehyde fixed asynchronous HeLa cells by ICC/IF. The antibody was incubated with the cells for 30 minutes and then bound antibody was detected using a Cy3 conjugated goat anti-rabbit antibody. Nuclei were visualised using DAPI staining. Ab19905 gives a pattern that is predominantly enriched within nuclei. This image is courtesy of an Abreview submitted by Kirk McManus.See Abreview

Staining of human tonsil tissue was performed using ab19905. Strong nuclear staining was observed in germinal center cells and scattered cells in the interfollicular area. T cells were weakly to moderately positive and endothelial cells were positive.

ab19905 (1/100) staining Dnmt1 in paraffin-embbeded mouse testis tissue sections. Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in 10mM citrate buffer pH 6.0 and blocking (30 minutes, 8% milk). A biotinylated anti-rabbit IgG (H+L) antibody was used as the secondary. For further experimental details please refer to abreview.See Abreview

developed using the ECL techniquePerformed under reducing conditions.

ICC/IF image of ab19905 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19905, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 1% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 1µg/ml.

Product References

The transcriptomic and proteomic effects of ectopic overexpression of miR-30d in - The transcriptomic and proteomic effects of ectopic overexpression of miR-30d in

Moreno-Moya JM, Vilella F, Martinez S, Pellicer A, Simon C. Mol Hum Reprod. 2014 Jun;20(6):550-66.

Class I HDACs are mediators of smoke carcinogen-induced stabilization of DNMT1 - Class I HDACs are mediators of smoke carcinogen-induced stabilization of DNMT1

Brodie SA, Li G, El-Kommos A, Kang H, Ramalingam SS, Behera M, Gandhi K, Kowalski J, Sica GL, Khuri FR, Vertino PM, Brandes JC. Cancer Prev Res (Phila). 2014 Mar;7(3):351-61. doi:

Epigenetic alterations in the brain associated with HIV-1 infection and - Epigenetic alterations in the brain associated with HIV-1 infection and

Desplats P, Dumaop W, Cronin P, Gianella S, Woods S, Letendre S, Smith D, Masliah E, Grant I. PLoS One. 2014 Jul 23;9(7):e102555.

Investigation of the expression patterns and correlation of DNA - Investigation of the expression patterns and correlation of DNA

Gu Y, Yang P, Shao Q, Liu X, Xia S, Zhang M, Xu H, Shao Q. Oncol Lett. 2013 Feb;5(2):452-458. Epub 2012 Dec 3.

A regulatory circuit of miR-148a/152 and DNMT1 in modulating cell transformation - A regulatory circuit of miR-148a/152 and DNMT1 in modulating cell transformation

Xu Q, Jiang Y, Yin Y, Li Q, He J, Jing Y, Qi YT, Xu Q, Li W, Lu B, Peiper SS, Jiang BH, Liu LZ. J Mol Cell Biol. 2013 Feb;5(1):3-13.

IL-6-induced DNMT1 activity mediates SOCS3 promoter hypermethylation in - IL-6-induced DNMT1 activity mediates SOCS3 promoter hypermethylation in

Li Y, Deuring J, Peppelenbosch MP, Kuipers EJ, de Haar C, van der Woude CJ. Carcinogenesis. 2012 Oct;33(10):1889-96.

Lentiviral transduction of CD34(+) cells induces genome-wide epigenetic - Lentiviral transduction of CD34(+) cells induces genome-wide epigenetic

Yamagata Y, Parietti V, Stockholm D, Corre G, Poinsignon C, Touleimat N, Delafoy D, Besse C, Tost J, Galy A, Paldi A. PLoS One. 2012;7(11):e48943.

Alpha-synuclein sequesters Dnmt1 from the nucleus: a novel mechanism for - Alpha-synuclein sequesters Dnmt1 from the nucleus: a novel mechanism for

Desplats P, Spencer B, Coffee E, Patel P, Michael S, Patrick C, Adame A, Rockenstein E, Masliah E. J Biol Chem. 2011 Mar 18;286(11):9031-7.

Distinct nuclear localization patterns of DNA methyltransferases in developing - Distinct nuclear localization patterns of DNA methyltransferases in developing

Nasonkin IO, Lazo K, Hambright D, Brooks M, Fariss R, Swaroop A. J Comp Neurol. 2011 Jul 1;519(10):1914-30.

Upregulation of DNA methyltransferase-mediated gene silencing, - Upregulation of DNA methyltransferase-mediated gene silencing,

Foran E, Garrity-Park MM, Mureau C, Newell J, Smyrk TC, Limburg PJ, Egan LJ. Mol Cancer Res. 2010 Apr;8(4):471-81.